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Technical question for MoBi people
Oct. 6th, 2008 11:09 amI'll pose the general question first, on the chance that more people might have opinions about it:
What forum(s) have you used to ask questions about technical molecular biology stuff? Were the answers to your questions useful? I'm taking a look at BioTechniques, Molecular Station (which doesn't looks very useful), and biowww.net as possibilities for posting the question below; I don't really want to spam a gazillion lists.
Second, the more specific question, which I somehow doubt most folks here will have an answer for, but I'm posting it anyway:
I'm cloning PCR products into Invitrogen's pCR8/GW/TOPO vector (a TA cloning vector with Gateway recombination sites) in their TA cloning kit. I've cloned about 8 things so far, and in two of them I've had the following problem: somewhere in the actual cloning, a few bases (9 in one case, 5 in a second) are removed from the end of the PCR product and the vector. Has anyone else had this problem? Recommendations on how to avoid it?
What forum(s) have you used to ask questions about technical molecular biology stuff? Were the answers to your questions useful? I'm taking a look at BioTechniques, Molecular Station (which doesn't looks very useful), and biowww.net as possibilities for posting the question below; I don't really want to spam a gazillion lists.
Second, the more specific question, which I somehow doubt most folks here will have an answer for, but I'm posting it anyway:
I'm cloning PCR products into Invitrogen's pCR8/GW/TOPO vector (a TA cloning vector with Gateway recombination sites) in their TA cloning kit. I've cloned about 8 things so far, and in two of them I've had the following problem: somewhere in the actual cloning, a few bases (9 in one case, 5 in a second) are removed from the end of the PCR product and the vector. Has anyone else had this problem? Recommendations on how to avoid it?
