amethyst73 | All ready!

I spent today organizing little tubes and making lists.

I've spent large portions of the last couple of months doing PCR mutagenesis and subcloning (and in the last couple of weeks, some RT-PCR too). I now have 40 different tubes of DNA to bring up to Berkeley to do sequencing. Once I get there and start doing transformations, they'll turn into 90 transformation tubes - that's, um, kind of a lot.

Those will turn into 40 agar plates with colonies, which will then turn into 4 96-well culture blocks, which will become 8 sequencing plates. I will be very busy while I'm there.

*sigh* Well, I did want to be really productive while there, so that I wouldn't have to go up too often.

And now it turns out that I may have to make up some antibiotic here to bring up as well. Maybe I should do that now so that I have it on hand if it turns out I need it. I can always store it in the freezer long-term if I end up not having to bring it to Berkeley.

Oh, and