Work: progressing!

[amethyst73]

I finally finished trawling through my miles of sequencing data today and started figuring out exactly where I stand with regards to the clones I'm after.  Turns out I'm much closer than I thought I was to having around a hundred of these buggers.  Here's my update, for anyone interested.

Genes that I want: 125

Clones that are picture-perfect (or close enough for my purposes) 79
Clones that are very close (reverse orientation, 1-2 errors to fix) 14

Those groups alone add up to 93.  (7 of the second group are straight fixits and should be very easy; the others are reverse orientation, sometimes with additional errors.)  A standard plate format is 96 wells. 

There's an additional 15 which require additional sequencing, most of which should fall neatly into one of the two above piles.  At which point, we're up above 100 successfully cloned genes - a target which somehow just kept feeling really really far away.  (The remaining genes have issues of various sorts - cloned the wrong thing when I did RT-PCR, don't see a band when I do RT-PCR (or sometimes standard PCR off a known template, etc.)

Comments

[nezumiko.livejournal.com]

AWESOME! Does this bode well for future Friday afternoons?

[amethyst73.livejournal.com]

Alas, no. This just means that I start thinking about - then doing - the next work-intensive part of the project. Free Fridays will still only be about one a month at best, due to the vacation days that I must spend.

[nezumiko.livejournal.com]

Well, we'll just have to content ourselves with those vacation Fridays then. Does it at least mean you can spend less time driving to Berkeley?

[amethyst73.livejournal.com]

That's... an excellent question. I'll still have to do it some, but hopefully the overall frequency will decrease. (I was advised this AM that I should spend the next month pushing to get the remaining genes on the list. I'll likely be going to Berkeley sometime in the next few weeks (to finish sequencing some longer clones) and can hopefully bring up a handful of new ones to do at the same time.. that way that can only be one trip. (Thinking aloud, sorry!) Then there will be the occasional trips up as I deal with the *next* set of genes we're going to try to work at... but hopefully I won't have to go much in between!